Laboratory for Extracellular Vesicle Research
- NTA analysis for determination of EV concentration and size: NanoSight NS300 (Malvern Panalytical)
- Ultracentrifugation for EV isolation: Ultracentrifuges Optima XPN 80 or Optima MAX-XP (both Beckman Coulter)
Contact: Metka Lenassi (metka.lenassi@mf.uni-lj.si)
Access: The equipment is open for community upon training or provided as service.
Department of Synthetic Biology and Immunology
- Ultracentrifuge L-100 XP, Beckman Coulter
- Spectral flow cytometer Aurora, Cytek
- Dynamic light scattering – DLS, Zetasizer HT, Malvern
Contact: for UC and DLS Mateja Manček Keber (mateja.mancek@ki.si), for Cytek Mojca Benčina (mojca.bencina@ki.si)
Access: The equipment is open for community upon training or provided as service.
Laboratory of Clinical Biophysics
The Laboratory is situated at the Department of Orthopaedic Surgery, University Medical Centre Ljubljana with the intention to be easily reached by the patients from different hospital buildings. Because the EV samples are sensitive to movement, the blood sampling can be performed in the same room where the processing and assessment take place. Two specialized collaborators are skilled for blood sampling.
Equipment for measurement of micro/nano vesicles:
- Light and fluorescent microscope ARISTOPLAN CLSM, Leica
- Flow cytometer MACS QUANT, Miltenyi
- Ultracentrifuge L8-70M, Beckman Coulter
- Centrifuge Centric 200R, Liliput swinging rotor (custom made by Domel)
- Ball-mill Millmix 20 with supplement equipment, Domel
Contact: Veronika Kralj-Iglič (kraljiglic@gmail.com), Marko Jeran (marko.jeran@fe.uni-lj.si), Aleš Iglič (ales.iglic@fe.uni-lj.si), Anna Romolo (annaromolo@gmail.com)
Access: Equipment is available in the context of collaboration for experiments that merit the ethical standards. Collaboration is welcome.
Institute of Cell Biology, Center for Electron Microscopy
For characterization of EVs we use methods of transmission electron microscopy, as are negative staining and ultrathin resin sections, and also methods of scanning electron microscopy. The proteins of EVs can be detected with immunolabelling on cryo-ultrathin sections. Larger EVs (>1 µm) are studied with fluorescence microscopy, phase-contrast microscopy in fixed cells or in living cells.
- Fluorescent microscope with Apotome device for optical sectioning (AxioImager, Zeiss)
- Scanning electron microscope (Vega, Tescan)
- Transmission electron microscope (CM100, Philips)
Contact: Peter Veranič (peter.veranic@mf.uni-lj.si)
Access: The equipment is open for community provided as service.
Laboratory: Cellular and molecular toxicology
- Flow cytometer Cell Lab QUANTA SC MPL; Beckman Coulter; bead-based flow cytometric assay
Contact: Katarina Černe (katarina.cerne@mf.uni-lj.si)
Access: In accordance with Article 45 of the Rules on the evaluation and financing of research and infrastructure programs. Preliminary agreement with the contact person.
Centre for Functional genomics and Bio-Chips
- NGS sequencers: MiniSeq (Illumina), GridION (Nanopore, the sequencer is part of ELIXIR-SI)
- Microarray technology: Affymetrix/Clariom chips, all glass slide microarrays (Agilent, etc)
- LC-MS (shared at IBK)
Contact: Tadeja Režen (tadeja.rezen@mf.uni-lj.si), Cene Skubic (cene.skubic@mf.uni-lj.si)
Access: The equipment is open for community upon training or provided as service.
Equipment for measurement of micro/nano vesicles:
- Set-up for synthesis/electroformation giant unillamelar vesicles (GUVs) (the main unit: Pt-electrod system coupled with alternating electric field generator Hameg Instruments)
- Anodization set-up for electrochemical synthesis of TiO2 nanostructured surfaces (fabricated by University of Erlangen) with the Voltcraft VSP 2653/VSP 2206 signal generator and the set-up for treatment of TiO2 nanostructures with gas plasma (fabricated by IJS Ljubljana)
- High-temperature furnace Nabertherm for the synthesis of nanoparticles and nanostructured surfaces
- System for computer controlled algae cultivation with incubation respirometer Echo, Echo
- Inverted light microscope Eclipse TE2000-S (Nikon EM CCD) coupled with digital camera system SPOT BOOST (Visitron Systems) and alternating magnetic field generator
Contact: Aleš Iglič (ales.iglic@fe.uni-lj.si), Marko Jeran (marko.jeran@fe.uni-lj.si)
Access: Equipment is available in the context of collaboration for experiments that merit the ethical standards. Collaboration is welcome.
SiN-EV network 